VOCATIONAL INSTRUCTOR IN MEDICAL LABORATORY

VOCATIONAL INSTRUCTOR IN MEDICAL 

LABORATORY TECHNICIAN COURSE 

VOCATIONAL HIGHER SECONDARY EDUCATION. 

(CATEGORY NO. 94/2011 & 96/2011) 

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1. Anatomy, Physiology & Biochemistry 
Anatomy
Anatomical and histological aspects of skeletal system, muscular system, 
joints, Thorax, Respiratory system, Heart, Vascular System, Lymphatic System, 
Digestive System, Urinary System, Respiratory System, Nervous System, Endocrine 
System, Skin and Tissues.
Physiology
Blood-composition and functions, respiratory system, Digestive system, 
cardiovascular system, renal system, endocrinology, reproductive system, muscle and 
nerve, nervous system, skin and temperature regulation.
Biochemistry
Units of measurements, laboratory glasswares, cleaning of glasswares, 
laboratory safety, laboratory hazards, first aid, expressing concentrations of solutions, 
grades of chemicals, purification of water, buffer systems, balance, centrifuge, 
calibration of glasswares, primary and secondary standards, Collection and 
preservation of biological specimens, colorimetry, spectrophotometry, flourimetry, 
nephalometry, flame photometry, radioisotopes. Physical chemistry - osmosis, 
diffusion, dialysis, Donnan-membrane equilibrium, viscosity, colloids, emulsions.
Basic knowledge about the metabolism of carbohydrate, protein, lipids, 

nucleic acid, vitamins, minerals, haemoglobin, prophyrins, enzymes, acid-base 
balance, nutrition, hormones. Function tests - liver, kidney, gastro intestinal, 
pancreas, gonadal, feto-placental, thyroid function tests. Lipid profile , analysis of 
calculi, electrophoresis, chromatographic techniques.
CSF analysis ,urine qualitative and quantitative analysis, Bence Jones Protein 
urine metabolite -VMA & 5- HIAA and gastric juice analysis. Quality control programme and automation in a clinical biochemistry laboratory.
Estimation of various parameters present in the blood ( glucose, urea, cholesterol, 
uric acid, creatinine ,phosphorous, calcium, electrolytes, Bilirubin ) samples and their 
normal values and interpretations, GCT, GTT, Glycosylated haemoglobin. Clinical 
enzymology - ALP, ACP, Amylase, AST, ALT, LDH, CK (activity determination, 
normal values, Interpretations etc).

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2. . Microbiology 
General Microbiology General Microbiology
History of microbiology, laboratory safety. Microscopy - compound, dark ground, fluorescent and electron microscope. Sterilization and disinfection - different agents and technique used. Staining techniques - Gram's, Acid fast & other special staining techniques. Morphological classification of bacteria, culture media, composition & preparation of media, culture technique, biochemical tests used for identifying bacteria, a study of medically important bacteria, processing clinical specimen for diagnosing infections, serological tests for diagnosis of infections, antibiotic susceptibility tests. 
Parasitology
Medically important protozoan and helminthic parasites and important protozoa, 
nematodes, cestodes and trematodes their morphology, life cycle and laboratory diagnosis. 
Different techniques used for the laboratory diagnosis of intestinal protozoan infections, 
Trichomoniasis, malaria, leishmaniasis, trypanosomiasis, filariasis , intestinal helminthic 
infections etc. 
Mycology
A brief study of common medically important fungi & their infections. Common contaminant 
fungi. Morphological study - KOH preparation, Lacto phenol cotton blue, slide culture, culture 
media - composition and preparation of Sabouraud's dextrose agar. 
Virology - Structure of viruses, DNA & RNA viruses, cultivation of viruses, common viral 
infections and laboratory diagnosis of infections. 
Immunology - Antigen, Antibody , Antigen-Antibody reactions - agglutination, precipitation, 
ELISA,IF and common serological techniques for diagnosing microbial infections

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3. Pathology 
Haematology - Composition of blood, development of blood cells. Normal and 
abnormal blood cell morphology, functions and identification. Haemoglobin -
function, normal and abnormal haemoglobins and estimation. Anaemia, Leukemia, 
blood coagulation factors and mechanism of coagulation, disorders of coagulation, 
anticoagulants, collection of specimens, RBC, WBC, platelets and absolute eosinophil 
count, DC, ESR, PCV, osmotic fragility, Romanowsky stain ,supra vital staining , 
reticulocyte count, red cell indices ( MCV, MCH, MCHC, CI). Bone marrow smear 
and staining. Peroxidase, PAS staining and Perl's staining and their significance. 
Blood parasites identification, LE cell preparation and identification. Blood 
coagulation studies and disorders of coagulation ( BT, CT, PT, PTT & TT ). 
Automatic blood cell counter. 
Clinical Pathology - Urine - normal and abnormal constituents, physical, chemical 
and microscopic examination, stool examination for parasites, sputum examination, 
semen analysis, pregnancy test, CSF examination, examination of other body fluids. 
Histotechnology - Introduction to histology. Reception of specimen, fixation, 
decalcification, processing of specimens (dehydration, clearing, infiltration and 
embedding). Microtomes and cutting of sections, staining and mounting 
(haemotoxylin & Eosin staining, PAS, Reticulin, VanGieson, Verhoeffs, Masson 
trichrome). Processing of frozen sections, Histokinet, Cryostat. 
Blood banking - ABO and other blood group system Rh system. Antigen antibody 
reactions in immune haematology. Anticoagulants used in blood bank. Preparation of 
grouping sera. Grouping and Rh typing of blood, screening of donors, collection, 
preservation and storage of blood. Compatibility test in blood transfusion, 
serological tests on donor blood. Transfusion reactions, transmission of diseases by 
blood transfusion. Component separation. 
Cytology - Important applications of cytology, collection of various specimens and 
preparation of smear, cell blocks, cytospin, cytological fixatives Papanicoloau's 
staining, Shorr's staining, identification of normal and abnormal smear, FNAC. 
Cytogenetics - Buccal smear preparation, staining and Barr body identification. 
Karyotyping - chromosome identification, abnormal chromosomes and chromosome 
defects.
NOTE: - It may be noted that apart from the topics detailed above, questions 
from other topics prescribed for the educational qualification of the post may 
also appear in the question paper. There is no undertaking that all the topics 
above may be covered in the question paper.

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